Art.No.

D05-210

Unit

5 Sample Test Kit , if tested in 2 strains (TA98, TA100), 4 replicates, 6 doses, +/- S9, negative, positive controls, sterility control. Not included in the kit: Rat liver S9 (5 x 1 mL), controls (4-NQO, 2-NF, 2-AA), microplates

Description

MicroAmes24 is a ready to use kit including strains, media, ampicillin, booster solution to run the Ames Test in a miniaturized format. Positive controls and rat liver S9 preparations can be chosen separately. Bacteria are exposed to different and low concentrations of a test sample, 6 mg/ strain, as well as a positive and a negative control in a medium containing limited quantities of histidine (S. typhimurium) to support approximately two cell divisions. After exposure, the cultures in each condition (negative control, test samples and positive controls) are poured in 24 well plates. Exposure is performed in absence and presence of S9 which mimics the effect of the chemical after metabolisation with rat liver post mitochondrial S9 fraction. Within three days, cells that have undergone reversion to amino acid prototrophy will grow and form colonies whose counts will be compared to those grown in the solvent (negative) control wells. A dose-dependent and significant increase in the number of revertant colonies upon exposure to test sample relative to the solvent controls indicates that the sample is mutagenic.

Service analytics for early drug discovery with test compound of 11 mg / strain (+/- S9) with the MicroAmes24 assay system is available as well under GLP and non GLP conditions, please contact This email address is being protected from spambots. You need JavaScript enabled to view it. .

Characteristics

• Quality controlled products: all products of the kit and in addition to the kit
• Certificate of Analysis: Provided
• Enough Material to process Strains individually, +/- S9
• Shipped at Room Temperature from external Storage in Germany
• 4 times less test compound as compared to the Ames Test on 10 cm agar plate test
• Less hands-on-time and simultaneous processing of several replicates
• 3Rs - 12 fold less consumption of rat liver liver S9, less test animals
• Waste - Significant less plastic waste and thus reduced contaminated waste in environment

To complete the MicroAmes24 Testkit, please add:

• Aroclor 1254 induced lyophilized rat liver S9, 1 mL (PRS-AC01),
• 2 mL (PRS-AC02)Phenobarbital/β-Naphtoflavone induced lyophilized rat liver S9,
• 1 mL (PRS-PB01),
• 2 ml (PRS-PB02)2-Nitrofluorene (2-NF),
• 20 μg, PPC-NF002-Aminoanthracene (2-AA),
• 100 μg, PPC-AA014-Nitroquinoline-N-oxide (4-NQO),
• 50 μg, PPC-NQ02S9 Cofactor Kit (Art.No. PCO-0800) for preparation of S9 Mix

Art.No.

C01-512-S2-P

Unit

5 x 48 Measuring Points: 1 Sample in 5 Strains (TA98, TA100, TA1535, TA1537, E.coli uvrA/E.coli pkM101) if tested in 3 Replicates, 6 Dilutions, +/- PB/ß-Naphtoflavone-induced S9, Negative and Positive Controls. Not included in the kit: S9 cofactor solutions, microplates

Description

Ready to use Starter Kit for Ames Test including 1 vial each of semisolid TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA, E.coli WP2 pKM101, Booster, Growth -, Exposure -, Reversion Indicator Media, Positive Controls, Post-Mitochondrial Supernatant S9 from lyophilized PB/ßNaphtoflavone induced rat liver.

Characteristics

• Quality controlled reagents: Strains, S9, media, positive controls - no inhouse quality control, no sterility control necessary   
• Certificate of Analysis is provided
• Excel Calculation Sheet to support data interpretation
• Number of tests corresponds to 5 x 480 agar plates.
• Standardized procedure
• Enough material to process strains individually
• Shipped at room temperature from external storage in Germany or from Switzerland
• High sensitivity and excellent correlation with agar plate test (see referenced publications)
• 4 times less test compound as compared to the agar plate test
• Less hands-on-time and simultaneous processing of several replicates
• Automation available - high throughput
• 3Rs - 12 fold less consumption of rat liver liver S9, significant less test animals sacrificed
• Less error prone due to easy colorimetric read-out
• Waste - Significant less plastic waste and thus reduced contaminated waste in environment

Art.No.

C01-512-S1-P

Unit

5 x 48 Measuring Points: 1 Sample in 5 strains (TA98, TA100, TA1535, TA1537, E.coli uvrA/E.coli pkM101) if tested in 3 Replicates, 6 Dilutions, +/- 1254 Aroclor-induced S9, Negative and Positive Controls. Not included in the kit: S9 cofactor solutions, microplates

Description

Ready to use Starter Kit for Ames Test including 1 vial each of semisolid TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA, E.coli WP2 pKM101, Booster, Growth -, Exposure -, Reversion Indicator Media, Positive Controls, Post-Mitochondrial Supernatant S9 from lyophilized Aroclor 1254 induced rat liver.

Characteristics

• Quality controlled reagents: Strains, S9, media, positive controls - no inhouse quality control, no sterility control necessary   
• Certificate of Analysis is provided
• Excel Calculation Sheet to support data interpretation
• Number of tests corresponds to 5 x 480 agar plates.
• Standardized procedure
• Enough material to process strains individually
• Shipped at room temperature from external storage in Germany or from Switzerland
• High sensitivity and excellent correlation with agar plate test (see referenced publications)
• 4 times less test compound as compared to the agar plate test
• Less hands-on-time and simultaneous processing of several replicates
• Automation available - high throughput
• 3Rs - 12 fold less consumption of rat liver liver S9, significant less test animals sacrificed
• Less error prone due to easy colorimetric read-out
• Waste - Significant less plastic waste and thus reduced contaminated waste in environment

Art.No.

C10-512-S1-P

Unit

5 x 480 Measuring points: 10 Samples in 5 strains (TA98, TA100, TA1535, TA1537, E.coli uvrA/E.coli pkM101) if tested in 3 Replicates, 6 Dilutions, +/- 1254 Aroclor-induced S9, Negative and Positive Controls. Not included in the kit: S9 cofactor solutions, microplates

Description

Ready to use Kit for Ames Test Kit including 10 vials each of semisolid TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA, E.coli WP2 pKM101, Booster, Growth -, Exposure -, Reversion Indicator Media, Positive Controls, Post-Mitochondrial Supernatant S9 from lyophilized Aroclor 1254 induced rat liver.

Characteristics

• Quality controlled reagents: All strains, S9, media, positive controls - no inhouse quality control, no sterility control necessary   
• Certificate of Analysis is provided
• Excel Calculation Sheet to support data interpretation
• Number of tests corresponds to 5 x 480 agar plates.
• Standardized procedure
• Enough material to process strains individually
• Shipped at room temperature from external storage in Germany or from Switzerland
• High sensitivity and excellent correlation with agar plate test (see referenced publications)
• 4 times less test compound as compared to the agar plate test
• Less hands-on-time and simultaneous processing of several replicates
• Automation available - high throughput
• 3Rs - 12 fold less consumption of rat liver liver S9, significant less test animals sacrificed
• Less error prone due to easy colorimetric read-out
• Waste - Significant less plastic waste and thus reduced contaminated waste in environment