Why Essence Line?

ESSENCE LINE   provides complete bionalytical services essential for monitoring human and   animal biomarkers, biomarker discovery and drug development.

In cooperation with academic institutes, we develop original analytical methods and apply them in practise using HPLC-MS/MS, NMR, RT-PCR, microarrays and Ames test.

1. Physico-Chemical Properties

UN Methods

• C.1 Metal Corrosivity (Chapter 37.4)     
• H.4 Heat accumulation storage test (Chapter 28.4.4)
• N.1 Test method for readily combustible solids (Chapter 33.2)
• N.2 Test method for pyrophoric solids (Chapter 33.3)
• N.3 Test method for pyrophoric liquids (Chapter 33.3)
• N.4 Test method for self-heating substances (Chapter 33.3)
• N.5 Test method for substances which in contact with  water emit flammable gases (Chapter 33.4)
• O.1 Test for oxidizing solids (Chapter 34)
• O.2 Test for oxidizing liquids (Chapter 34)

CIPAC Methods

• CIPAC MT 22          Viscosity
• CIPAC MT 39          Stability of liquid formulations at 0°C
• CIPAC MT 46          Accelerated Storage Procedure
• CIPAC MT 47          Persistent Foaming
• CIPAC MT 53          Wettability
• CIPAC MT 75          pH and pH at 1%
• CIPAC MT 148         Pourability
• CIPAC MT 160         Spontaneity of dispersion
• CIPAC MT 171         Dustiness
• CIPAC MT 172         Flowability of water dispersible granules
• CIPAC MT 174         Dispersibility
• CIPAC MT 176         Dissolution of water soluble bag
• CIPAC MT 178         Attrition resistance of dispersible granules
• CIPAC MT 179         Dissolution degree and solution stability
• CIPAC MT 184         Suspensibility of formulations
• CIPAC MT 185         Wet sieve test
• CIPAC MT 191         Free Acidity and Alkalinity
  

2. In Vitro-Toxicology

Skin corrosion

• OECD 431               In vitro Skin Corrosion (EpiDerm)
• OECD 435               Membrane Barrier Test- Corrosit

Skin irritation

• OECD 439               In vitro Skin Irritation (EpiDerm)

Skin sensitization

• OECD 442C            Direct Peptide Reactivity Assay (DPRA)

Eye irritation

• OECD 437               Bovine Corneal Opacity and Permeability-BCOP Test
• In vitro Eye Irritation [HET CAM]
• Human EpiOcularTM Eye Irritation Test (EIT)

Cytotoxicity

• ISO 10993-5                Growth Inhibition Test

3. In Vivo-Toxicology

Acute Toxicity 

• B.1 tris / OECD 423  Acute oral Toxicity on rats
• B.3 / OECD 402       Acute dermal Toxicity on rats
• B.2 / OECD 403       Inhalation Toxicity on rats
• B.4 / OECD 404       Skin Irritation on rabbits
• B.5 / OECD 405       Eye Irritation on rabbits
• B.6 / OECD 406       Sensibilisation
• [Magnusson & Kligman / Bühler]
• OECD 429               Local Lymph Node Assay [LLNA]
• OECD 442B             Skin Sensitization: Local Lymph Node  Assay: BrdU-ELISA
• OECD 442D            In Vitro Skin Sensitization (ARE reporter gene assay)

Chronic, Subchronic, Reproduction and Carcinogenicity Toxicity

• B.7 / OECD 407        28 days Toxicity on rats
• B.8 / OECD 408        90 days Toxicity on rats
• B.31 / OECD 414      Prenatal Development Toxicity
• B.35 / OECD 416      2 Generation Study
• OECD 421                Screening for Reproduction Toxicity
• OECD 422                Combined Repeated Dose Toxicity  with Reproduction Toxicity
• OECD 443                Extended One-Generation  Reproductive Toxicity Study

4. Mutagenicity / Genotoxicity

In vitro - Studies

• OECD 471                Ames Test /Ames Screening Test (MPF Test;  Xenometrix)
• OECD 473                Chromosome Aberration in human  lymphocytes
• OECD 487                Micronucleus in human lymphocytes
• OECD 490                Mouse Lymphoma Assay
• OECD 476                HPRT- Hypoxanthine-guanine phosphoribosyl  transferase
• ASTM E1687            Modified Ames Test - Standard Test Method for Determining Carcinogenic Potential of Virgin Base Oils in Metalworking Fluids

5. Biodegradability

Ready Biodegradability

• OECD 301A              DOC-Die-Away Test
• OECD 301B              CO₂-Evolution Test (Sturm Test)
• OECD 301C              Modified MITI Test (I)
• OECD 301D              Closed-Bottle Test
• OECD 301E              Modified OECD Screening Test
• OECD 301F              Manometric Respirometry Test
• OECD310                 CO₂-Headspace Test

Inherent Biodegradation

• OECD 302B              Zahn-Wellens-Test
• OECD 302C              Modified MITI-Tes

Anaerobe Biodegradation

• OECD 311 / EN ISO 11734 Anaerobic degradation

Special biodegradation

• OECD 303A               Activated Sludge Units
• DEKRA PSR              Plant Growth Test with Cress - Toxicity determination of oil degradation products

 6. Environmental Fate

• OECD 303               Simulation Test – Aerobic Sewage Treatment
• OECD 305               Bioaccumulation (Danio rerio) - aquatic study  - feed study
• OECD 306               Biodegradability in Seawater
• CEC L-103-12          Degradation of Lubricants with GC-Analysis

14C-radiolabelled and non radiolabelled* 

• OECD 307                Aerobic and Anaerobic Transformation in  Soil
• OECD 308                Aerobic and Anaerobic Transformation in  Aquatic Sediment Systems
• OECD 309                Aerobic Mineralisation in Surface Water – Simulation Biodegradation

7. Ecotoxicology

Aquatic Studies:

Acute Studies

• OECD 201                Toxicity on Alga (72h) (Desmodesmus,  Pseudokirchneriella, Cyanobacteria)
• OECD 202                Toxicity on Daphnia (24/48h)
• OECD 203                Toxicity on Fish (96h) (Danio rerio.  Onchorhynchus mykiss/Rainbow trout)
• OECD 236                Fish Embryo Acute Toxicity (FET) Test    (Danio rerio)
• OECD DRP (2008)   Fish Life-Cycle Test (Danio rerio)

Long Term Studies 

• OECD 204                Prolonged fish toxicity (14d/28d; Danio rerio)
• OECD 210                Early Life Stage Toxicity (Danio rerio)
• OECD 211                Reproduction-Toxicity (Daphnia magna)
• OECD 214                Short Term Toxicity Test Embryo/Sac-fry
• OECD 215                Juvenile Growth Test (Danio rerio)
• Fish Life-cycle          Test (Danio rerio)

Bacteria Inhibition Test

• OECD 209:               Anaerobi Activated sludge respiration inhibition  testing
• OECD 224:               Anaerobic Inhibition of the activity of  anaerobic bacteria
• ISO 9509                  Nitrification Inhibition Study

Terrestrial Studies

• OECD 207                Earthworm, Acute Toxicity Tests (Eisenia fetida; Eisenia andrei)
• OECD 208                Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test
• OECD 222                Earthworm Reproduction Test (Eisenia fetida;  Eisenia andrei)
• OECD 216                Nitrogen Transformation Test
• OECD 217                Carbon Transformation Test
• OECD 218                Sediment-Water Chironomid Toxicity Test  using spiked sediment
• OECD 219                Sediment-Water Chironomid Toxicit Test   using spiked water
• OECD 225                Sediment Water Lumbriculus Test
• OECD 233                Sediment-Water Chironomid Life-Cycle T  oxicity Test Using Spiked Water or Spiked Sediment
• OECD 235                Chironomus sp., Acute Immobilisation Test

8. Analytical Instrumentation

Chemicals

Acc. REACH

• Physico-chemical Properties
• in vitro / in vivo* - Toxicology
• Mutagenicity / Genotoxicity
• Aquatic and Terrestrial Ecotoxicology
• Environmental Fate and Behaviour
• Endocrine Disruptors

Sameness acc. REACH

• GPC *
• UV/VIS
• IR
• 1H-NMR/13C-NMR/solid NMR *
• Mass spectrum:
• GC/MS with Headspace and LC-MS/MS
• GC/HPLC Chromatogram
• ICP-OES
• XRF *
• XRD *
• Titration
• Water content:
• Gravimetric, Karl Fischer, freeze drying*

Analytical chemistry

• UV-VIS
• DSC
• FTIR
• Liquid-Scintillation-Counter
• ICP-OES
• TOC
• Titration unit

Chromatography

• GC - FID, ECD, NPD
• GC-MS - EI, Headspace
• HPLC - UV, RI, FLD, ELSD, RAD
• HPLC-MS - ESI, APCI
• LC-MS/MS

9. Endocrine Disruptors

Level 1  Sorting & prioritization based upon existing information

Level 2  In vitro assays providing mechanistic data

Level 3  In vivo assays providing data about single endocrine mechanisms

Level 4  In vivo assays providing data about multiple endocrine mechanisms

Level 5  In vivo assays providing adverse effects data from endocrine & other mechanisms for RA

Level 2 

• Estrogen or androgen receptor binding affinity (YES/YAS Assay)
• Stereoidogenesis in vitro (OECD TG 456)*
• MCF-7 cell proliferation assays (ER ant/agonist)*
• Other assays as appropriate*

Level 3

• Uterotrophic assay (OECD TG 440)*
• Hershberger assay (OECD TG 441)*

Level 4

• Repeated dose studies (OECD407/408)*
• Prenatal developmental toxicity study (OECD 414)*
• Chronic toxicity and carcinogenicity studies (OECD 451-3)*
• Reproductive screening test (OECD 421)*
• Combined 28-day/reproductive screening assay (OECD 422)*
• Fish sexual development test (OECD 234)
• Fish Reproduction Partial Lifecycle Test
• Chironomid Toxicity Test (OECD 218-219)
• Daphnia Reproduction Test (OECD 211)
• Earthworm Reproduction Test (OECD 222)
• Sediment Water Lumbriculus Toxicity Test (OECD 225)

Level 5

• Fish Life Cycle Test (Danio rerio)