XenoScreen YES YAS - Estrogen, Androgen Screening Test for Endocrine Disruptive Chemicals EDC

Art.No.

N05-233-Y

Unit

5 Samples in 4 receptor activities (estrogen, androgen, agonistic and antagonistic activity) if tested in duplicates, 8 dilutions. 10 samples can be measured for agonistic activity only. 40 samples in 4 receptor activities in duplicates, if only 1 dilution is measured. YES and YAS strains (up to 20 passages) as well as YES YAS Culture Maintenance Set need to be added separately.

Description

XenoScreen YES YAS is a ready to use estrogen and androgen receptor transactivation test kit for the identification of chemicals, agrochemicals, cosmetics (EDC's) or water samples with endocrine disruptive activity, i.e estrogenic and androgenic activity or antagonistic activity. In the receptor transactivation test a chemical is required to bind to the receptor and to activate the receptor. In compliance with OECD Guideline TG455 and ISO 19040. The kit can also be used for measurement of agonistic activity only allowing the measurement of 8 compounds.
Many endocrine disrupting chemicals adversely impact the estrogen or androgen signaling by interacting with estrogen or androgen receptors. The receptors show ligand selectivity and target gene regulation, they modulate metabolic enzymes that are critical for normal estrogen or androgen synthesis and metabolism. Typical EDC's are e.g. phytoestrogens, bisphenolics, and organochlorine pesticides. Many endocrine discrupting chemicals act through multiple mechanisms and therefore biological effects of EDCs need to be carefully interpreted.
In the receptor transcriptional activation assay, YES (Yeast Estrogen Screen) and YAS (Yeast Androgen Screen) cells are exposed to 8 concentrations of a test sample, as well as a positive and a negative control. After exposure, the receptor mediated β-galactosidase expression is assessed via a chromogenic substrate. Each dose is tested in duplicate to allow for statistical analysis of the data.
A dose-dependent and significant increase or decrease in the color development relative to the corresponding negative control upon exposure indicates that the sample acts as an endocrine disruptor. Comparison with standardized positive controls (tested in multiple doses) allows for a quantification of the endocrine disrupting properties in terms of EC10, EC50 of EEQ/AEQ.

Characteristics

• Includes all media, controls, also quality controlled plastic ware
• Number of samples: 5 samples in 4 different receptors (ER, AR, Agonist, Antagonist), in duplicates, 8 dilutions or for 40 samples in 4 different receptors in 1 dilution. Allows for measurement of only 1 receptor activity whereby 4 times more samples can be measrued.
• Excel calculation sheet is available free of charge to support result interpretation
• Does not include Lyticase
• Strains are not included in the kit and are available separately.